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Title: Inactivation of Clostridium difficile spores by microwave irradiation
Authors: Suvash Chandra Ojha
Surang Chankhamhaengdecha
Sombat Singhakaew
Puey Ounjai
Tavan Janvilisri
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Apr-2016
Citation: Anaerobe. Vol.38, (2016), 14-20
Abstract: © 2015 Elsevier Ltd. Spores are a potent agent for Clostridium difficile transmission. Therefore, factors inhibiting spores have been of continued interest. In the present study, we investigated the influence of microwave irradiation in addition to conductive heating for C. difficile spore inactivation in aqueous suspension. The spores of 15 C. difficile isolates from different host origins were exposed to conductive heating and microwave irradiation. The complete inhibition of spore viability at 107CFU/ml was encountered following microwave treatment at 800 W for 60 s, but was not observed in the conductive-heated spores at the same time-temperature exposure. The distinct patterns of ultrastructural alterations following microwave and conductive heat treatment were observed and the degree of damages by microwave was in the exposure time-dependent manner. Microwave would therefore be a simple and time-efficient tool to inactivate C. difficile spores, thus reducing the risk of C. difficile transmission.
ISSN: 10958274
Appears in Collections:Scopus 2016-2017

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