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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/40848
Title: B cell responses during secondary dengue virus infection are dominated by highly cross-reactive, memory-derived plasmablasts
Authors: Lalita Priyamvada
Alice Cho
Nattawat Onlamoon
Nai Ying Zheng
Min Huang
Yevgeniy Kovalenkov
Kulkanya Chokephaibulkit
Nasikarn Angkasekwinai
Kovit Pattanapanyasat
Rafi Ahmed
Patrick C. Wilson
Jens Wrammert
Emory University School of Medicine
Emory University
Mahidol University
University of Chicago
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 1-Jun-2016
Citation: Journal of Virology. Vol.90, No.12 (2016), 5574-5585
Abstract: © 2016, American Society for Microbiology. Dengue virus (DENV) infection results in the production of both type-specific and cross-neutralizing antibodies. While immunity to the infecting serotype is long-lived, heterotypic immunity wanes a few months after infection. Epidemiological studies link secondary heterotypic infections with more severe symptoms, and cross-reactive, poorly neutralizing antibodies have been implicated in this increased disease severity. To understand the cellular and functional properties of the acute dengue virus B cell response and its role in protection and immunopathology, we characterized the plasmablast response in four secondary DENV type 2 (DENV2) patients. Dengue plasmablasts had high degrees of somatic hypermutation, with a clear preference for replacement mutations. Clonal expansions were also present in each donor, strongly supporting a memory origin for these acutely induced cells. We generated 53 monoclonal antibodies (MAbs) from sorted patient plasmablasts and found that DENV-reactive MAbs were largely envelope specific and cross neutralizing. Many more MAbs neutralized DENV than reacted to envelope protein, emphasizing the significance of virion-dependent B cell epitopes and the limitations of envelope protein-based antibody screening. A majority of DENV-reactive MAbs, irrespective of neutralization potency, enhanced infection by antibody-dependent enhancement (ADE). Interestingly, even though DENV2 was the infecting serotype in all four patients, several MAbs from two patients neutralized DENV1 more potently than DENV2. Further, half of all type-specific neutralizing MAbs were also DENV1 biased in binding. Taken together, these findings are reminiscent of original antigenic sin (OAS), given that the patients had prior dengue virus exposures. These data describe the ongoing B cell response in secondary patients and may further our understanding of the impact of antibodies in dengue virus pathogenesis.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84971449627&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/40848
ISSN: 10985514
0022538X
Appears in Collections:Scopus 2016-2017

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