Please use this identifier to cite or link to this item:
|Title:||Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures|
T. Eoin West
Udon Thani Regional Medical Sciences Center
University of Washington, Seattle
|Keywords:||Immunology and Microbiology|
|Citation:||Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.110, No.11 (2016), 670-672|
|Abstract:||© The Author 2016. Background: Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis. Methods: We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016. Results: Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p < 0.0001. Conclusions: The IFA accurately expedites the diagnosis of melioidosis.|
|Appears in Collections:||Scopus 2016-2017|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.