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dc.contributor.authorWarobon Noppakunmongkolchaien_US
dc.contributor.authorTeera Poyomtipen_US
dc.contributor.authorThichakorn Jittawuttipokaen_US
dc.contributor.authorNatthanej Luplertlopen_US
dc.contributor.authorAnavaj Sakuntabhaien_US
dc.contributor.authorSarin Chimnaronken_US
dc.contributor.authorSiwanon Jirawatnotaien_US
dc.contributor.authorRutaiwan Tohtongen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherInstitut Pasteur, Parisen_US
dc.contributor.otherCNRS Centre National de la Recherche Scientifiqueen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.date.accessioned2018-12-11T03:04:50Z
dc.date.accessioned2019-03-14T08:01:48Z-
dc.date.available2018-12-11T03:04:50Z
dc.date.available2019-03-14T08:01:48Z-
dc.date.issued2016-03-01en_US
dc.identifier.citationVirology Journal. Vol.13, No.1 (2016)en_US
dc.identifier.issn1743422Xen_US
dc.identifier.other2-s2.0-84978731786en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84978731786&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/40877-
dc.description.abstract© 2016 Noppakunmongkolchai et al. Background: Dengue virus (DENV) is a member of the Flaviviridae family, transmitted to human via mosquito. DENV infection is common in tropical areas and occasionally causes life-threatening symptoms. DENV contains a relatively short positive-stranded RNA genome, which encodes ten viral proteins. Thus, the viral life cycle is necessarily rely on or regulated by host factors. Methods: In silico analyses in conjunction with in vitro kinase assay were used to study kinases that potentially phosphorylate DENV NS5. Potential kinase was inhibited or activated by a specific inhibitor (or siRNA), or an activator. Results of the inhibition and activation on viral entry/replication and host cell survival were examined. Results: Our in silico analyses indicated that the non-structural protein 5 (NS5), especially the RNA-dependent RNA polymerase (RdRp) domain, contains conserved phosphorylation sites for protein kinase C (PKC). Phosphorylation of NS5 RdRp was further verified by PKC in vitro kinase assay. Inhibitions of PKC by a PKC-specific chemical inhibitor or siRNA suppressed NS5 phosphorylation in vivo, increased viral replication and reduced viability of the DENV-infected cells. In contrary, activation of PKC effectively suppressed intracellular viral number. Conclusions: These results indicated that PKC may act as a restricting mechanism that modulates the DENV replication and represses the viral outburst in the host cells.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84978731786&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleInhibition of protein kinase C promotes dengue virus replicationen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1186/s12985-016-0494-6en_US
Appears in Collections:Scopus 2016-2017

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