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dc.contributor.authorAuttawit Sirichoaten_US
dc.contributor.authorAroonlug Lulitanonden_US
dc.contributor.authorRattiyaporn Kanlayaen_US
dc.contributor.authorRatree Tavichakorntrakoolen_US
dc.contributor.authorAroonwadee Chanawongen_US
dc.contributor.authorSujintana Wongthongen_US
dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.otherKhon Kaen Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationDiagnostic Microbiology and Infectious Disease. Vol.86, No.4 (2016), 340-344en_US
dc.description.abstract© 2016 Elsevier Inc. Reduced vancomycin susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem. Unfortunately, its genetic marker and molecular mechanisms remained unknown. This study investigated differential phenotypic characteristic and protein expression profiles among three groups of MRSA isolates, including vancomycin-susceptible S. aureus (VSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) (n = 7 isolates/group). Phenotypic characteristic revealed significant greater number of isolates with non-spreading colony in VISA as compared to both VSSA and hVISA groups. 2-DE followed by nanoLC-MS/MS analyses revealed increased glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in both hVISA and VISA, whereas 50S ribosomal protein L14 (RplN) and DNA-binding protein II (Hup) were increased only in VISA. The non-spreading colony and GAPDH level of MRSA may be used as the markers for differentiation of VSSA, hVISA and VISA.en_US
dc.rightsMahidol Universityen_US
dc.titlePhenotypic characteristics and comparative proteomics of Staphylococcus aureus strains with different vancomycin-resistance levelsen_US
Appears in Collections:Scopus 2016-2017

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