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Title: A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus
Authors: Pranav Patel
Ahmed Abd El Wahed
Oumar Faye
Pauline Prüger
Marco Kaiser
Sasikanya Thaloengsok
Sukathida Ubol
Anavaj Sakuntabhai
Isabelle Leparc-Goffart
Frank T. Hufert
Amadou A. Sall
Manfred Weidmann
Matthias Niedrig
Robert Koch Institut
Universität Göttingen
Institut Pasteur de Dakar
GenExpress GmbH
Mahidol University
Institut Pasteur, Paris
Institute for Biomedical Research of the French Armed Forces (IRBA)
Brandenburg Medical School
University of Stirling
Keywords: Medicine
Issue Date: 29-Sep-2016
Citation: PLoS Neglected Tropical Diseases. Vol.10, No.9 (2016)
Abstract: © 2016 Patel et al. Background: Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. Methodology/Principal Findings: In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. Conclusions/Significance: The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.
ISSN: 19352735
Appears in Collections:Scopus 2016-2017

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