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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41164
Title: Skin scrapings versus standardized skin surface biopsy to detect Demodex mites in patients with facial erythema of uncertain cause - A comparative study
Authors: Sumanas Bunyaratavej
Chuda Rujitharanawong
Pranee Kasemsarn
Waranya Boonchai
Chanai Muanprasert
Lalita Matthapan
Charussi Leeyaphan
Mahidol University
Keywords: Medicine
Issue Date: 1-Sep-2016
Citation: Indian Journal of Dermatology, Venereology and Leprology. Vol.82, No.5 (2016), 519-522
Abstract: © 2016 Indian Journal of Dermatology, Venereology, and Leprology Published by Wolters Kluwer - Medknow. Background: Standardized skin surface biopsy (SSSB) is considered to be the gold standard technique to evaluate the density of Demodex mites for the diagnosis of demodicidosis. Potassium hydroxide (KOH) preparation of skin scrapings is a much simpler procedure that can be used to detect pathogens in the superficial skin. Objective: To evaluate the reliability of potassium hydroxide preparation of skin scrapings as compared to the standard skin biopsy technique with regard to capacity to detect Demodex mites, time consumed and technician satisfaction. Methods: One hundred outpatients presenting with facial erythema of uncertain cause were enrolled. Standardized skin surface biopsy and potassium hydroxide preparation of skin scrapings were undertaken in adjacent areas on the patients' right cheek. Limitation: Patients with normal facial skin were excluded from the study. Results: The accuracy of Demodex mite detection by potassium hydroxide preparation of skin-scrapings when compared to the standard procedure is 82%. The sensitivity, specificity, positive and negative predictive values of this method are 75%, 84.2%, 60% and 91.43%, respectively. There was no statistically significant difference between the standard and skin scraping techniques (P = 0.238) with regard to mite detection. Mean preparation time while using the skin scraping technique was 6 times less than that of the standard technique. For interpretation also, skin scraping technique (3.6 min) consumed much less time than the biopsy technique (9.8 min). Moreover, experienced technicians were more satisfied with skin scraping. Conclusion: Potassium hydroxide preparation of skin scrapings is an effective, time saving and practical technique to detect Demodex mites with accuracy comparable to the standard biopsy method.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84983684895&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41164
ISSN: 09733922
03786323
Appears in Collections:Scopus 2016-2017

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