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Title: Comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents
Authors: Theodore Gouliouris
Beth Blane
Hayley J. Brodrick
Kathy E. Raven
Kirsty E. Ambridge
Angela D. Kidney
Nazreen F. Hadjirin
M. Estée Török
Direk Limmathurotsakul
Sharon J. Peacock
University of Cambridge
Public Health England
Cambridge University Hospitals NHS Foundation Trust
Mahidol University
London School of Hygiene & Tropical Medicine
Keywords: Medicine
Issue Date: 1-Aug-2016
Citation: Diagnostic Microbiology and Infectious Disease. Vol.85, No.4 (2016), 409-412
Abstract: © 2016 The Authors We compared ChromID VRE and Brilliance VRE media for the detection of vancomycin-resistant enterococci (VRE). Using a panel of 28 enterococcal isolates, 10 vanA Enterococcus faecium and three vanA Enterococcus faecalis isolates grew as per manufacturers’ instructions whilst growth of two vanC and eight vancomycin-susceptible enterococci was inhibited on both media. Important differences were noted in the selectivity and chromogenic properties of the two media for vanA Enterococcus raffinosus and vanB E. faecium. The two media were further evaluated using 295 stool samples from nursing home residents, 34 of which grew VRE (11.5%). ChromID and Brilliance had comparable sensitivity, which was increased markedly by prolonging incubation to 48 hours (from 29% to 82%, and from 41% to 85%, respectively) and by a pre-enrichment step (to 97% and 100%, respectively). Brilliance VRE agar had higher selectivity at 48 hours, and after pre-enrichment.
ISSN: 18790070
Appears in Collections:Scopus 2016-2017

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