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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41294
Title: Rapid and Sensitive Multiplex Detection of Burkholderia pseudomallei-Specific Antibodies in Melioidosis Patients Based on a Protein Microarray Approach
Authors: Christian Kohler
Susanna J. Dunachie
Elke Müller
Anne Kohler
Kemajittra Jenjaroen
Prapit Teparrukkul
Vico Baier
Ralf Ehricht
Ivo Steinmetz
Friedrich-Loeffler-Institute
Mahidol University
University of Oxford
Alere Technologies GmbH
InfectoGnostics Research Campus
Sappasitthiprasong Hospital
Medizinische Universitat Graz
Keywords: Medicine
Issue Date: 18-Jul-2016
Citation: PLoS Neglected Tropical Diseases. Vol.10, No.7 (2016)
Abstract: © 2016 Kohler et al. Background: The environmental bacterium Burkholderia pseudomallei causes the infectious disease melioidosis with a high case-fatality rate in tropical and subtropical regions. Direct pathogen detection can be difficult, and therefore an indirect serological test which might aid early diagnosis is desirable. However, current tests for antibodies against B. pseudomallei, including the reference indirect haemagglutination assay (IHA), lack sensitivity, specificity and standardization. Consequently, serological tests currently do not play a role in the diagnosis of melioidosis in endemic areas. Recently, a number of promising diagnostic antigens have been identified, but a standardized, easy-to-perform clinical laboratory test for sensitive multiplex detection of antibodies against B. pseudomallei is still lacking. Methods and Principal Findings: In this study, we developed and validated a protein microarray which can be used in a standard 96-well format. Our array contains 20 recombinant and purified B. pseudomallei proteins, previously identified as serodiagnostic candidates in melioidosis. In total, we analyzed 196 sera and plasmas from melioidosis patients from northeast Thailand and 210 negative controls from melioidosis-endemic and non-endemic regions. Our protein array clearly discriminated between sera from melioidosis patients and controls with a specificity of 97%. Importantly, the array showed a higher sensitivity than did the IHA in melioidosis patients upon admission (cut-off IHA titer ≥1:160: IHA 57.3%, protein array: 86.7%; p = 0.0001). Testing of sera from single patients at 0, 12 and 52 weeks post-admission revealed that protein antigens induce either a short- or long-term antibody response. Conclusions: Our protein array provides a standardized, rapid, easy-to-perform test for the detection of B. pseudomallei-specific antibody patterns. Thus, this system has the potential to improve the serodiagnosis of melioidosis in clinical settings. Moreover, our high-throughput assay might be useful for the detection of anti-B. pseudomallei antibodies in epidemiological studies. Further studies are needed to elucidate the clinical and diagnostic significance of the different antibody kinetics observed during melioidosis.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84980383431&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41294
ISSN: 19352735
19352727
Appears in Collections:Scopus 2016-2017

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