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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41302
Title: Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children
Authors: Catrin E. Moore
Kristin Elwin
Nget Phot
Chanthou Seng
Saroeun Mao
Kuong Suy
Varun Kumar
Johanna Nader
Rachel Bousfield
Sanuki Perera
J. Wendi Bailey
Nicholas J. Beeching
Nicholas P.J. Day
Christopher M. Parry
Rachel M. Chalmers
Mahidol University
Angkor Hospital for Children
Nuffield Department of Clinical Medicine
Singleton Hospital
Swansea University
Liverpool School of Tropical Medicine
Addenbrooke's Hospital
University of Liverpool
London School of Hygiene & Tropical Medicine
Nagasaki University
Keywords: Medicine
Issue Date: 7-Jul-2016
Citation: PLoS Neglected Tropical Diseases. Vol.10, No.7 (2016)
Abstract: © 2016 Moore et al. Background: In a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays. Methodology/Principal Findings: Cryptosporidium oocysts were detected in 2.2% (11/498) of samples using microscopy and in 7.7% (38/498) with molecular tests. Giardia duodenalis cysts were detected in 18.9% (94/498) by microscopy and 27.7% (138/498) by molecular tests; 82% of the positive samples (by either method) were from children aged 1–10 years. Cryptosporidium hominis was the most common species of Cryptosporidium, detected in 13 (34.2%) samples, followed by Cryptosporidium meleagridis in 9 (23.7%), Cryptosporidium parvum in 8 (21.1%), Cryptosporidium canis in 5 (13.2%), and Cryptosporidium suis and Cryptosporidium ubiquitum in one sample each. Cryptosporidium hominis and C. parvum positive samples were subtyped by sequencing the GP60 gene: C. hominis IaA16R6 and C. parvum IIeA7G1 were the most abundant subtypes. Giardia duodenalis was typed using a multiplex real-time PCR targeting assemblages A and B. Assemblage B (106; 76.8% of all Giardia positive samples) was most common followed by A (12.3%) and mixed infections (5.1%). Risk factors associated with Cryptosporidium were malnutrition (AOR 9.63, 95% CI 1.67–55.46), chronic medical diagnoses (AOR 4.51, 95% CI 1.79–11.34) and the presence of birds in the household (AOR 2.99, 95% CI 1.16–7.73); specifically C. hominis (p = 0.03) and C. meleagridis (p<0.001) were associated with the presence of birds. The use of soap was protective against Giardia infection (OR 0.74, 95% CI 0.58–0.95). Conclusions/Significance: This is the first report to describe the different Cryptosporidium species and subtypes and Giardia duodenalis assemblages in Cambodian children. The variety of Cryptosporidium species detected indicates both anthroponotic and zoonotic transmission in this population. Interventions to improve sanitation, increase hand washing after defecation and before preparing food and promote drinking boiled water may reduce the burden of these two parasites.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84980325561&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41302
ISSN: 19352735
19352727
Appears in Collections:Scopus 2016-2017

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