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Title: Comparative proteomics analysis of Neisseria gonorrhoeae strains in response to extended-spectrum cephalosporins
Authors: Sunanta Nabu
Ratana Lawung
Patcharee Isarankura-Na-Ayudhya
Sittiruk Roytrakul
Supamas Dolprasit
Sineenart Sengyee
Chartchalerm Isarankura-Na-Ayudhya
Virapong Prachayasittikul
Mahidol University
Thammasat University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 8-Nov-2017
Citation: EXCLI Journal. Vol.16, (2017), 1207-1229
Abstract: © 2017, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved. Neisseria gonorrhoeae strains displaying reduced susceptibility and resistance to extended-spectrum cephalosporins (ESCs) are major public health concerns. Although resistance mechanisms of ESCs have extensively been studied, the proteome-wide investigation on the biological response to the antibiotic stress is still limited. Herein, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS analysis was applied to investigate the global protein expression under ESC stresses of ESC-susceptible and ESCreduced susceptible N. gonorrhoeae strains. Upon exposure to ceftriaxone, 14 and 21 proteins of ESCsusceptible and ESC-reduced susceptible strains, respectively, were shown to be differentially expressed. In the meanwhile, differential expressions of 13 and 17 proteins were detected under cefixime stress for ESCsusceptible and ESC-reduced susceptible strains, respectively. ESC antibiotics have been proven to trigger the expression of several proteins implicated in a variety of biological functions including transport system, energy metabolism, stress response and pathogenic virulence factors. Interestingly, macrophage infectivity potentiators (Ng-MIP) showed increased expression for ESC-reduced susceptible strain under ESC stress. The altered expression of Ng-MIP was found to be a unique response to ESC stresses. Our finding proposes a broad view on proteomic changes in N. gonorrhoeae in response to ESC antibiotics that provides further insights into the gonococcal antimicrobial resistance and physiological adaptation mechanism.
ISSN: 16112156
Appears in Collections:Scopus 2016-2017

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