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Title: A filtration-based rapid test using a partially purified third-stage larval antigen to detect specific antibodies for the diagnosis of gnathostomiasis
Authors: A. Ma
Y. Wang
X. L. Liu
H. M. Zhang
P. Eamsobhana
H. S. Yong
X. X. Gan
Zhejiang Academy of Medical Sciences
Chinese Center for Disease Control and Prevention
Mahidol University
University of Malaya
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 16-Nov-2017
Citation: Journal of Helminthology. (2017), 1-7
Abstract: Copyright © Cambridge University Press 2017 Human gnathostomiasis is an emerging food-borne parasitic disease caused by nematodes of the genus Gnathostoma. Currently, serological tests are commonly applied to support clinical diagnosis. In the present study, a simple and rapid filtration-based test, dot immune–gold filtration assay (DIGFA) was developed using a partially purified antigen of Gnathostoma third-stage larvae (L3). A total of 180 serum samples were tested to evaluate the diagnostic potential of DIGFA for gnathostomiasis. The diagnostic sensitivity and specificity were 96.7% (29/30) and 100% (25/25), respectively. The cross-reactivity with sera from other helminthiasis patients ranged from 0 to 4%, with an average of 1.6% (2/125). DIGFA using a partially purified L3 antigen was not only simple and rapid, but also more accurate than standard assays for the diagnosis of human gnathostomiasis. DIGFA may represent a promising tool for application in laboratories or in the field, without requiring any instrumentation.
ISSN: 14752697
Appears in Collections:Scopus 2016-2017

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