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dc.contributor.authorSayera Banuen_US
dc.contributor.authorSuporn Pholwaten_US
dc.contributor.authorSuporn Foongladdaen_US
dc.contributor.authorRattapha Chinlien_US
dc.contributor.authorDuangjai Boonlerten_US
dc.contributor.authorSara Sabrina Ferdousen_US
dc.contributor.authorS. M.Mazidur Rahmanen_US
dc.contributor.authorArfatur Rahmanen_US
dc.contributor.authorShahriar Ahmeden_US
dc.contributor.authorEric R. Houpten_US
dc.contributor.otherInternational Centre for Diarrhoeal Disease Research Bangladeshen_US
dc.contributor.otherUniversity of Virginiaen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-21T06:30:15Z
dc.date.accessioned2019-03-14T08:02:23Z-
dc.date.available2018-12-21T06:30:15Z
dc.date.available2019-03-14T08:02:23Z-
dc.date.issued2017-05-01en_US
dc.identifier.citationPLoS ONE. Vol.12, No.5 (2017)en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-85019131030en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019131030&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/41424-
dc.description.abstract© 2017 Banu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Culture based phenotypic drug susceptibility testing (DST) for Mycobacterium tuberculosis (TB) is time consuming therefore rapid genotypic methods are increasingly being utilized. We previously developed and evaluated on TB isolates a rapid genotypic TaqMan array card (TAC) that detects mutations in several resistance-associated genes using dozens of primer pairs, probes, and high resolution melt analysis, with >96% accuracy versus Sanger sequencing. In this study we examined the performance of TAC on sputum, comparing results between 71 paired sputum and TB isolates of which 62 were MDR-TB. We also adapted the TAC to include wild-type probes and broadened coverage for rpoB and gyrA mutations. TAC was 89% successful at detecting wild-type or mutations within inhA, katG, rpoB, eis, gyrA, rplC, and pncA on smear positive sputa and 33% successful on smear negative sputa. The overall accuracy of these detections as compared to the TAC results of the paired isolate was 95% ± 7 (average sensitivity 98% ± 3; specificity 92% ± 14). Accuracy of sputum TAC results versus phenotypic DST for isoniazid, rifampin, ofloxacin/moxifloxacin, and pyrazinamide was 85% ± 12. This was similar to that of the isolate TAC results (accuracy 88% ± 13), thus inaccuracies primarily reflected intrinsic genotypic-phenotypic discordance. The TAC is a rapid, modular, comprehensive, and accurate TB DST for the major first and second line TB drugs and could be used for supplemental testing of GeneXpert resistant smear positive sputum.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019131030&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titlePerformance of TaqMan array card to detect TB drug resistance on direct specimensen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1371/journal.pone.0177167en_US
Appears in Collections:Scopus 2016-2017

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