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Title: Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
Authors: Sittichai Boonrawd
Ravi Mani
Sirikwan Ponprateep
Premruethai Supungul
Promsin Masrinoul
Anchalee Tassanakajon
Vichien Rimphanitchayakit
Chulalongkorn University
Sathyabama Institute of Science and Technology
Srinakharinwirot University
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Keywords: Agricultural and Biological Sciences;Environmental Science
Issue Date: 1-Jun-2017
Citation: Fish and Shellfish Immunology. Vol.65, (2017), 88-95
Abstract: © 2017 Elsevier Ltd Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway.
ISSN: 10959947
Appears in Collections:Scopus 2016-2017

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