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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41487
Title: Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples
Authors: Hiroki Hashizume
Megumi Sato
Marcello Otake Sato
Sumire Ikeda
Tippayarat Yoonuan
Surapol Sanguankiat
Tiengkham Pongvongsa
Kazuhiko Moji
Toshifumi Minamoto
Kobe University
Niigata University
Dokkyo Medical University
Mahidol University
Savannakhet Provincial Health Department
Nagasaki University
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology
Issue Date: 1-May-2017
Citation: Acta Tropica. Vol.169, (2017), 1-7
Abstract: © 2017 Elsevier B.V. Opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in Southeast Asian countries. The trematode, Opisthorchis viverrini, is the causative agent of the disease. Accurate and rapid monitoring of O. viverrini is crucial for disease prevention and containment. Therefore, in this study we sought to develop a novel species-specific real-time PCR assay for detecting O. viverrini using environmental DNA (eDNA). The diagnostic sensitivity of the newly developed real-time PCR assay was similar to that of the traditional PCR assay for 50 fecal samples collected in Lao PDR (21 and 19 samples were positive by real-time PCR and traditional PCR, respectively). The efficacy of eDNA analysis and its applicability in the field were tested using a total of 94 environmental water samples collected from 44 sites in Savannakhet, Lao PDR during May and October 2015 and February 2016. O. viverrini eDNA was detected in five samples by real-time PCR, indicating the presence of the fluke in the area and the risk of infection for individuals consuming fish from these water sources. The application of eDNA analysis would facilitate the identification of O. viverrini endemic hotspots and contribute to the ecological control of opisthorchiasis, and this strategy can be applied to other eukaryotic water pathogens.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010280787&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41487
ISSN: 18736254
0001706X
Appears in Collections:Scopus 2016-2017

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