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dc.contributor.authorHiroki Hashizumeen_US
dc.contributor.authorMegumi Satoen_US
dc.contributor.authorMarcello Otake Satoen_US
dc.contributor.authorSumire Ikedaen_US
dc.contributor.authorTippayarat Yoonuanen_US
dc.contributor.authorSurapol Sanguankiaten_US
dc.contributor.authorTiengkham Pongvongsaen_US
dc.contributor.authorKazuhiko Mojien_US
dc.contributor.authorToshifumi Minamotoen_US
dc.contributor.otherKobe Universityen_US
dc.contributor.otherNiigata Universityen_US
dc.contributor.otherDokkyo Medical Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherSavannakhet Provincial Health Departmenten_US
dc.contributor.otherNagasaki Universityen_US
dc.identifier.citationActa Tropica. Vol.169, (2017), 1-7en_US
dc.description.abstract© 2017 Elsevier B.V. Opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in Southeast Asian countries. The trematode, Opisthorchis viverrini, is the causative agent of the disease. Accurate and rapid monitoring of O. viverrini is crucial for disease prevention and containment. Therefore, in this study we sought to develop a novel species-specific real-time PCR assay for detecting O. viverrini using environmental DNA (eDNA). The diagnostic sensitivity of the newly developed real-time PCR assay was similar to that of the traditional PCR assay for 50 fecal samples collected in Lao PDR (21 and 19 samples were positive by real-time PCR and traditional PCR, respectively). The efficacy of eDNA analysis and its applicability in the field were tested using a total of 94 environmental water samples collected from 44 sites in Savannakhet, Lao PDR during May and October 2015 and February 2016. O. viverrini eDNA was detected in five samples by real-time PCR, indicating the presence of the fluke in the area and the risk of infection for individuals consuming fish from these water sources. The application of eDNA analysis would facilitate the identification of O. viverrini endemic hotspots and contribute to the ecological control of opisthorchiasis, and this strategy can be applied to other eukaryotic water pathogens.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleApplication of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samplesen_US
Appears in Collections:Scopus 2016-2017

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