Please use this identifier to cite or link to this item:
|Title:||Lipid and protein oxidation levels in spermatozoa and seminal plasma of Asian Elephants (Elephas maximus) and their relationship with semen parameters|
King Mongkut's University of Technology North Bangkok
Thailand Forest Industry Organization
|Keywords:||Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology|
|Citation:||Reproduction in Domestic Animals. Vol.52, No.2 (2017), 283-288|
|Abstract:||© 2017 Blackwell Verlag GmbH Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility. Results revealed that seminal plasma malondialdehyde (MDA) and seminal plasma protein carbonyls (PCs) were significantly higher in poor motility than in good motility (p <.05). The MDA and PC levels in seminal plasma were negatively correlated with the percentages of progressive motility (p <.05). In addition, the negative correlation between sperm concentration and seminal plasma MDA level was investigated (p <.05). The sperm viability was also negatively correlated with sperm PC level (p <.05). This study indicated that lipid and protein oxidation has deleterious effect on semen quality of Asian elephants.|
|Appears in Collections:||Scopus 2016-2017|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.