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Title: Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei
Authors: Andre Göhler
Trinh Thanh Trung
Verena Hopf
Christian Kohler
Jörg Hartleib
Vanaporn Wuthiekanun
Sharon J. Peacock
Direk Limmathurotsakul
Apichai Tuanyok
Ivo Steinmetz
Medizinische Universität Graz
Vietnam National University Hanoi
Ernst-Moritz-Arndt-Universität Greifswald
Mahidol University
London School of Hygiene & Tropical Medicine
University of Florida
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Environmental Science;Immunology and Microbiology
Issue Date: 1-Apr-2017
Citation: Applied and Environmental Microbiology. Vol.83, No.8 (2017)
Abstract: © 2017 American Society for Microbiology. All Rights Reserved. Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment are a prerequisite for assessing the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a quantitative PCR (qPCR) targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from a comparative genomic analysis. Two hundred soil samples from two rice paddies in northeast Thailand were evaluated, of which 47% (94/200) were B. pseudomallei culture positive. The TTSS1 qPCR and two novel qPCR assays that targeted open reading frames (ORFs) BPSS0087 and BPSS0745 exhibited detection rates of 76.5% (153/200), 34.5% (69/200), and 74.5% (150/200), respectively. The combination of TTSS1 and BPSS0745 qPCR increased the detection rate to 90% (180/200). Combining the results of the three qPCR assays and the BPSS1187 nested PCR previously published, all 200 samples were positive by at least one PCR assay. Samples positive by either TTSS1 (n = 153) or BPSS0745 (n = 150) qPCR were more likely to be direct-culture positive, with odds ratios of 4.0 (95% confidence interval [CI], 1.7 to 9.5; P < 0.001) and 9.0 (95% CI, 3.1 to 26.4; P < 0.001), respectively. High B. pseudomallei genome equivalents correlated with high CFU counts by culture. In conclusion, multitarget qPCR improved the B. pseudomallei detection rate in soil samples and predicted culture positivity. This approach has the potential for use as a sensitive environmental screening method for B. pseudomallei.
ISSN: 10985336
Appears in Collections:Scopus 2016-2017

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