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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41602
Title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species
Authors: Vichaya Suttisunhakul
Apinya Pumpuang
Peeraya Ekchariyawat
Vanaporn Wuthiekanun
Mindy G. Elrod
Paul Turner
Bart J. Currie
Rattanaphone Phetsouvanh
David A.B. Dance
Direk Limmathurotsakul
Sharon J. Peacock
Narisara Chantratita
Mahidol University
Faculty of Medicine, Thammasat University
National Center for Emerging and Zoonotic Infectious Diseases
Angkor Hospital for Children
Nuffield Department of Clinical Medicine
Royal Darwin Hospital
Menzies School of Health Research
Mahosot Hospital
University of Oxford
London School of Hygiene & Tropical Medicine
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Apr-2017
Citation: PLoS ONE. Vol.12, No.4 (2017)
Abstract: © 2017 Suttisunhakul et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates. Main spectral profiles (MSP) for 26 isolates of B. pseudomallei (N = 5) and other Burkholderia species (N = 21) were added to the Biotyper database. MALDI-TOF MS was then performed on 581 B. pseudomallei, 19 B. mallei, 6 B. thailandensis and 23 isolates representing a range of other bacterial species. B. pseudomallei originated from northeast and east Thailand (N = 524), Laos (N = 12), Cambodia (N = 14), Hong Kong (N = 4) and Australia (N = 27). All 581 B. pseudomallei were correctly identified, with 100% sensitivity and specificity. Accurate identification required a minimum inoculum of 5 × 107 CFU/ml, and identification could be performed on spiked blood cultures after 24 hours of incubation. Comparison between a dendrogram constructed from MALDI-TOF MS main spectrum profiles and a phylogenetic tree based on recA gene sequencing demonstrated that MALDI-TOF MS distinguished between B. pseudomallei and B. mallei, while the recA tree did not. MALDI-TOF MS is an accurate method for the identification of B. pseudomallei, and discriminates between this and other related Burkholderia species.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85017112646&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41602
ISSN: 19326203
Appears in Collections:Scopus 2016-2017

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