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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/41655
Title: Deciphering minimal antigenic epitopes associated with Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharide O-antigens
Authors: Marielle Tamigney Kenfack
Marcelina Mazur
Teerapat Nualnoi
Teresa L. Shaffer
Abba Ngassimou
Yves Blériot
Jérôme Marrot
Roberta Marchetti
Kitisak Sintiprungrat
Narisara Chantratita
Alba Silipo
Antonio Molinaro
David P. AuCoin
Mary N. Burtnick
Paul J. Brett
Charles Gauthier
Universite de Poitiers
Uniwersytet Przyrodniczy we Wroclawiu
University of Nevada School of Medicine
Prince of Songkla University
University of South Alabama
Institut Lavoisier de Versailles
Università degli Studi di Napoli Federico II
Mahidol University
INRS-Institut Armand Frappier
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 1-Dec-2017
Citation: Nature Communications. Vol.8, No.1 (2017)
Abstract: © 2017 The Author(s). Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), the etiologic agents of melioidosis and glanders, respectively, cause severe disease in both humans and animals. Studies have highlighted the importance of Bp and Bm lipopolysaccharides (LPS) as vaccine candidates. Here we describe the synthesis of seven oligosaccharides as the minimal structures featuring all of the reported acetylation/methylation patterns associated with Bp and Bm LPS O-antigens (OAgs). Our approach is based on the conversion of an l-rhamnose into a 6-deoxy-l-talose residue at a late stage of the synthetic sequence. Using biochemical and biophysical methods, we demonstrate the binding of several Bp and Bm LPS-specific monoclonal antibodies with terminal OAg residues. Mice immunized with terminal disaccharide-CRM197 constructs produced high-titer antibody responses that crossreacted with Bm-like OAgs. Collectively, these studies serve as foundation for the development of novel therapeutics, diagnostics, and vaccine candidates to combat diseases caused by Bp and Bm.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85025836405&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/41655
ISSN: 20411723
Appears in Collections:Scopus 2016-2017

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