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dc.contributor.authorYuma Ishidaen_US
dc.contributor.authorYuka Okamotoen_US
dc.contributor.authorYuta Matsuokaen_US
dc.contributor.authorArisa Tadaen_US
dc.contributor.authorJindaporn Janprasiten_US
dc.contributor.authorMayumi Yamatoen_US
dc.contributor.authorNoppawan Phumala Moralesen_US
dc.contributor.authorKen Ichi Yamadaen_US
dc.contributor.otherKyushu Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationFree Radical Biology and Medicine. Vol.113, (2017), 487-493en_US
dc.description.abstract© 2017 Elsevier Inc. Oxidized low density lipoprotein (Ox-LDL) is implicated in a variety of oxidative diseases. To clarify the mechanisms involved and facilitate the investigation of therapeutics, we previously developed a detection method for lipid-derived radicals using the fluorescent probe 2,2,6-trimethyl-6-pentyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)piperidine-1-oxyl (NBD-Pen). In this study, NBD-Pen was used to detect lipid-derived radicals in Ox-LDL from in vitro and in vivo samples using an iron overloaded mouse model. By following the timeline of lipid radical generation using this method, the iron overloaded mice could be successfully treated with the antioxidant Trolox, resulting in successful lowering of the plasma lipid peroxidation, aspartate transaminase and alanine transaminase levels. Furthermore, using a combination therapy of the chelating agent deferoxamine (DFX) and Trolox, liver injury and oxidative stress markers were also reduced in iron overloaded mice. The NBD-Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleDetection and inhibition of lipid-derived radicals in low-density lipoproteinen_US
Appears in Collections:Scopus 2016-2017

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