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Title: Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium
Authors: Pranee Inprakhon
Natcha Wongthongdee
Taweechai Amornsakchai
Thunyarat Pongtharankul
Panya Sunintaboon
Lars O. Wiemann
Alain Durand
Volker Sieber
Mahidol University
Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB
Laboratoire de Chimie Physique Macromoléculaire
Université de Lorraine
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 10-Oct-2017
Citation: Journal of Biotechnology. Vol.259, (2017), 182-190
Abstract: © 2017 Elsevier B.V. Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7 M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2 M sucrose monoester within 1 h at 50 °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.
ISSN: 18734863
Appears in Collections:Scopus 2016-2017

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