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Title: Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D
Authors: Warunya Chakritbudsabong
Ladawan Sariya
Sirikron Pamonsupornvichit
Rassmeepen Pronarkngver
Somjit Chaiwattanarungruengpaisan
Joao N. Ferreira
Piyathip Setthawong
Praopilas Phakdeedindan
Mongkol Techakumphu
Theerawat Tharasanit
Sasitorn Rungarunlert
Mahidol University
National University of Singapore, Faculty of Dentistry
Chulalongkorn University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Oct-2017
Citation: Stem Cell Research. Vol.24, (2017), 21-24
Abstract: © 2017 The Authors Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.
ISSN: 18767753
Appears in Collections:Scopus 2016-2017

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