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dc.contributor.authorArada Vinaiphaten_US
dc.contributor.authorSiripat Aluksanasuwanen_US
dc.contributor.authorJuthatip Manissornen_US
dc.contributor.authorSuchitra Sutthimethakornen_US
dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationProteomics. Vol.17, No.15-16 (2017)en_US
dc.description.abstract© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim We have previously identified changes in the cellular proteome of renal tubular cells induced by low-dose (100 μg/mL) and high-dose (1000 μg/mL) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals. However, the functional significance of such expression data remained unclear. In this study, we performed comparative analyses and functional investigations of four proteomic datasets to define potential mechanisms by which renal tubular cells responded to differential crystal types and doses. The data showed that high-dose induced greater changes than low-dose, whereas COM induced more changes than COD. Luciferin–luciferase ATP assay revealed increased intracellular ATP level by high-dose of both COM and COD. OxyBlot assay and Western blotting showed accumulated intracellular oxidized proteins but decreased ubiquitinated proteins by high-dose of both crystals. Flow cytometric analysis of cell death showed that high-dose of both crystals, particularly COM, significantly increased cell death. Also, crystal adhesion assay showed higher degree of cell–crystal adhesion in high-dose and COM when compared to low-dose and COD, respectively. Finally, pretreatment of epigallocatechin-3-gallate revealed a protective effect on COM/COD crystals-induced oxidative stress and cell-crystal adhesion. Collectively, these data may provide a better understanding of cellular responses of renal tubular cells to COM/COD crystals in kidney stone disease.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleResponse of renal tubular cells to differential types and doses of calcium oxalate crystals: Integrative proteome network analysis and functional investigationsen_US
Appears in Collections:Scopus 2016-2017

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