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Title: Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study
Authors: Panithi Sukho
Jolle Kirpensteijn
Jan Willem Hesselink
Gerjo J.V.M. van Osch
Femke Verseijden
Yvonne M. Bastiaansen-Jenniskens
Utrecht University
Erasmus University Medical Center
Mahidol University
Hill’s Pet Nutrition Inc
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Apr-2017
Citation: Stem Cell Reviews and Reports. Vol.13, No.2 (2017), 267-277
Abstract: © 2017, The Author(s). Adipose tissue-derived stem cells (ASCs) are known to be able to promote repair of injured tissue via paracrine factors. However, the effect of cell density and inflammatory cytokines on the paracrine ability of ASCs remains largely unknown. To investigate these effects, ASCs were cultured in 8000 cells/cm2, 20,000 cells/cm2, 50,000 cells/cm2, and 400,000 cells/cm2with and without 10 or 20 ng/ml tumor necrosis factor alpha (TNFα) and 25 or 50 ng/ml interferon gamma (IFNγ). ASC-sheets formed at 400,000 cells/cm2after 48 h of culture. With increasing concentrations of TNFα and IFNγ, ASC-sheets with 400,000 cells/cm2had increased production of angiogenic factors Vascular Endothelial Growth Factor and Fibroblast Growth Factor and decreased expression of pro-inflammatory genes TNFA and Prostaglandin Synthase 2 (PTGS2) compared to lower density ASCs. Moreover, the conditioned medium of ASC-sheets with 400,000 cells/cm2stimulated with the low concentration of TNFα and IFNγ enhanced endothelial cell proliferation and fibroblast migration. These results suggest that a high cell density enhances ASC paracrine function might beneficial for wound repair, especially in pro-inflammatory conditions.
ISSN: 15586804
Appears in Collections:Scopus 2016-2017

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