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Title: An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells
Authors: Kongtana Trakarnsanga
Rebecca E. Griffiths
Marieangela C. Wilson
Allison Blair
Timothy J. Satchwell
Marjolein Meinders
Nicola Cogan
Sabine Kupzig
Ryo Kurita
Yukio Nakamura
Ashley M. Toye
David J. Anstee
Jan Frayne
University of Bristol
Mahidol University
NHS Blood and Transplant
Japanese Red Cross Medical Center
Riken BioResource Center
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 14-Mar-2017
Citation: Nature Communications. Vol.8, (2017)
Abstract: © 2017 The Author(s). With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.
ISSN: 20411723
Appears in Collections:Scopus 2016-2017

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