Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/42013
Title: Development and Validation of Voriconazole Concentration by LC-MS-MS: Applied in Clinical Implementation
Authors: Santirat Prommas
Apichaya Puangpetch
Nuttawut Jenjirattithigarn
Sumonrat Chuwongwattana
Thawinee Jantararoungtong
Napatrupron Koomdee
Siwalee Santon
Montri Chamnanphon
Chonlaphat Sukasem
Mahidol University
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Health Professions
Issue Date: 1-Jan-2017
Citation: Journal of Clinical Laboratory Analysis. Vol.31, No.1 (2017)
Abstract: © 2016 Wiley Periodicals, Inc. Background: Voriconazole (VRZ) is a triazole antifungal used for treatment of invasive fungal infection, which is a life-threatening condition. Therapeutic drug monitoring is recommended for identifying the optimal dose in patients who have hepatic/renal impairment or reduced function of the CYP2C19 metabolizing enzyme. Methods: One hundred microliters of sample plasma was extracted by protein precipitated with 200 μl of acetonitrile containing fluconazole as internal standard (IS). After vortexing and centrifugation, supernatant was dried and reconstituted with 100 μl of mobile phase (ACN: 0.1% formic acid in 10 mM Ammonium acetate) (50:50 v/v) before injected. The column was C18, 2.7 μm, 3.0 × 50 mm at flow rate of 0.5 ml/min with retention time of 0.5 and 0.75 min for VRZ and IS, respectively. The tandem mass spectrometer was set in multiple reactions monitoring (MRM) mode with the following transition; VRZ m/z 350.10→281.10 and 307.20→220.20 (IS). Results: The accuracy and precision inter- and intra-day were less than 9%, over the range 0.05–10 μg/ml. The linearity was consistent (r2 = 0.9987) and recovery was more than 85.0% for both analyses. Conclusion: This method is applicable for routine monitoring of patients’ VRZ plasma level with fast and accurate runtime to assess CYP2C19 genotype.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994175817&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/42013
ISSN: 10982825
08878013
Appears in Collections:Scopus 2016-2017

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.