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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/42018
Title: C-terminal domain on the outer surface of the Macrobrachium rosenbergii nodavirus capsid is required for Sf9 cell binding and internalization
Authors: Monsicha Somrit
Atthaboon Watthammawut
Charoonroj Chotwiwatthanakun
Puey Ounjai
Wanida Suntimanawong
Wattana Weerachatyanukul
Mahidol University
Srinakharinwirot University
Thailand Ministry of Education
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 2-Jan-2017
Citation: Virus Research. Vol.227, (2017), 41-48
Abstract: © 2016 Elsevier B.V. We have shown that Macrobrachium rosenbergii nodavirus (MrNV) was able to infect Sf9 cells and that MrNV virus-like particles (MrNV-VLPs) were capable nanocontainers for delivering nucleic acid-based materials. Here, we demonstrated that chymotryptic removal of a C-terminal peptide and its truncated variant (F344-MrNV-VLPs) exhibited a drastically reduced ability to interact and internalize into Sf9 cells. Electron microscopic observations revealed that the loss of C-terminal domain either from enzyme hydrolysis or genetic truncation did not affect the generated MrNV-VLPs’ icosahedral conformation, but did drastically affect the VLPs’ internalization ability into Sf9 cells. Homology-based modelling of the MrNV capsid with other icosahedral capsid models revealed that this chymotrypsin-sensitive C-terminal domain was not only exposed on the capsid surface, but also constituted the core of the viral capsid protrusion. These results therefore suggest the importance of the C-terminal domain as a structure for targeted cell interaction which is presumably localized at the protruding domain. This work thus provided the functional insights into the role of the MrNV C-terminal domain in viral entry into Sf9 cells and lead to the development of strategies in combatting MrNV infection in susceptible cells.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84992166449&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/42018
ISSN: 18727492
01681702
Appears in Collections:Scopus 2016-2017

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