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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/42173
Title: SERS-based immunoassay on 2D-arrays of Au@Ag core-shell nanoparticles: influence of the sizes of the SERS probe and sandwich immunocomplex on the sensitivity
Authors: Kullavadee Karn-Orachai
Kenji Sakamoto
Rawiwan Laocharoensuk
Suwussa Bamrungsap
Tararaj Dharakul
Kazushi Miki
National Institute for Materials Science Tsukuba
University of Tsukuba
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Chemical Engineering;Chemistry
Issue Date: 1-Jan-2017
Citation: RSC Advances. Vol.7, No.23 (2017), 14099-14106
Abstract: © The Royal Society of Chemistry. We report a sandwich-type SERS-based immunoassay using a two-dimensional (2D) array of gold core@silver shell (Au@Ag) nanoparticles (NPs) as the SERS substrate and antibody-conjugated gold NPs labeled with 4-mercaptobenzoic acid (MBA) as the SERS probes. To achieve highly sensitive detection, the size of the SERS probes was first optimized for the immunoassay of Human-IgG (H-IgG), where the Au core size of the SERS probes was varied from 26 to 110 nm in diameter. The maximum SERS intensity was observed at an Au core size of 53 nm. Then, the influence of the size of the sandwich immunocomplexes on the sensitivity was examined by performing sandwich SERS immunoassays for H-IgG and prostate-specific antigen (PSA) using SERS probes with 53 nm Au core size. The sensitivity improvement by using the SERS substrate (2D-array of Au@Ag NPs) instead of an Au evaporated film, which was used as a reference substrate, was evaluated for each immunoassay. The sensitivity improvement for H-IgG and PSA detection was 2.3-fold and 6.4-fold, respectively. The larger sensitivity improvement for the PSA system can be attributed to the smaller immunocomplex of PSA; the shorter separation distance between the SERS probes and the SERS substrate induces stronger plasmon coupling. This result indicates that the sensitivity of the sandwich-type immunoassay performed on the SERS substrate increases with decreasing size of sandwich immunocomplex, suggesting that the sensitivity can be improved by adopting an antibody-fragment with the same affinity for the target antigen as that of the antibody.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85014342095&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/42173
ISSN: 20462069
Appears in Collections:Scopus 2016-2017

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