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dc.contributor.authorKullavadee Karn-Orachaien_US
dc.contributor.authorKenji Sakamotoen_US
dc.contributor.authorRawiwan Laocharoensuken_US
dc.contributor.authorSuwussa Bamrungsapen_US
dc.contributor.authorTararaj Dharakulen_US
dc.contributor.authorKazushi Mikien_US
dc.contributor.otherNational Institute for Materials Science Tsukubaen_US
dc.contributor.otherUniversity of Tsukubaen_US
dc.contributor.otherThailand National Science and Technology Development Agencyen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-21T07:07:32Z
dc.date.accessioned2019-03-14T08:03:12Z-
dc.date.available2018-12-21T07:07:32Z
dc.date.available2019-03-14T08:03:12Z-
dc.date.issued2017-01-01en_US
dc.identifier.citationRSC Advances. Vol.7, No.23 (2017), 14099-14106en_US
dc.identifier.issn20462069en_US
dc.identifier.other2-s2.0-85014342095en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85014342095&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/42173-
dc.description.abstract© The Royal Society of Chemistry. We report a sandwich-type SERS-based immunoassay using a two-dimensional (2D) array of gold core@silver shell (Au@Ag) nanoparticles (NPs) as the SERS substrate and antibody-conjugated gold NPs labeled with 4-mercaptobenzoic acid (MBA) as the SERS probes. To achieve highly sensitive detection, the size of the SERS probes was first optimized for the immunoassay of Human-IgG (H-IgG), where the Au core size of the SERS probes was varied from 26 to 110 nm in diameter. The maximum SERS intensity was observed at an Au core size of 53 nm. Then, the influence of the size of the sandwich immunocomplexes on the sensitivity was examined by performing sandwich SERS immunoassays for H-IgG and prostate-specific antigen (PSA) using SERS probes with 53 nm Au core size. The sensitivity improvement by using the SERS substrate (2D-array of Au@Ag NPs) instead of an Au evaporated film, which was used as a reference substrate, was evaluated for each immunoassay. The sensitivity improvement for H-IgG and PSA detection was 2.3-fold and 6.4-fold, respectively. The larger sensitivity improvement for the PSA system can be attributed to the smaller immunocomplex of PSA; the shorter separation distance between the SERS probes and the SERS substrate induces stronger plasmon coupling. This result indicates that the sensitivity of the sandwich-type immunoassay performed on the SERS substrate increases with decreasing size of sandwich immunocomplex, suggesting that the sensitivity can be improved by adopting an antibody-fragment with the same affinity for the target antigen as that of the antibody.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85014342095&origin=inwarden_US
dc.subjectChemical Engineeringen_US
dc.subjectChemistryen_US
dc.titleSERS-based immunoassay on 2D-arrays of Au@Ag core-shell nanoparticles: influence of the sizes of the SERS probe and sandwich immunocomplex on the sensitivityen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1039/c7ra00154aen_US
Appears in Collections:Scopus 2016-2017

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