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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/42210
Title: Synthesis and Immunological Studies of the Lipomannan Backbone Glycans Found on the Surface of Mycobacterium tuberculosis
Authors: Harin Leelayuwapan
Niwat Kangwanrangsan
Runglawan Chawengkirttikul
Marisa Ponpuak
Ratthaphol Charlermroj
Kanokthip Boonyarattanakalin
Somsak Ruchirawat
Siwarutt Boonyarattanakalin
Chulabhorn Research Institute
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
King Mongkut's Institute of Technology Ladkrabang
Sirindhorn International Institute of Technology, Thammasat University
Keywords: Chemistry
Issue Date: 21-Jul-2017
Citation: Journal of Organic Chemistry. Vol.82, No.14 (2017), 7190-7199
Abstract: © 2017 American Chemical Society. Investigations into novel bacterial drug targets and vaccines are necessary to overcome tuberculosis. Lipomannan (LM), found on the surface of Mycobacterium tuberculosis (Mtb), is actively involved in the pathogenesis and survival of Mtb. Here, we report for the first time a rapid synthesis and biological activities of an LM glycan backbone, α(1-6)mannans. The rapid synthesis is achieved via a regio- and stereoselective ring opening polymerization to generate multiple glycosidic bonds in one simple chemical step, allowing us to finish assembling the defined polysaccharides of 5-20 units within days rather than years. Within the same pot, the polymerization is terminated by a thiol-linker to serve as a conjugation point to carrier proteins and surfaces for immunological experiments. The synthetic glycans are found to have adjuvant activities in vivo. The interactions with DC-SIGN demonstrated the significance of α(1-6)mannan motif present in LM structure. Moreover, surface plasmon resonance (SPR) showed that longer chain of synthetic α(1-6)mannans gain better lectin's binding affinity. The chemically defined components of the bacterial envelope serve as important tools to reveal the interactions of Mtb with mammalian hosts and facilitate the determination of the immunologically active molecular components.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85025170933&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/42210
ISSN: 15206904
00223263
Appears in Collections:Scopus 2016-2017

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