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dc.contributor.authorKiatnida Treerattrakoonen_US
dc.contributor.authorWarangkana Chanthimaen_US
dc.contributor.authorChayachon Apiwaten_US
dc.contributor.authorTararaj Dharakulen_US
dc.contributor.authorSuwussa Bamrungsapen_US
dc.contributor.otherThailand National Science and Technology Development Agencyen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-12-21T07:10:45Z
dc.date.accessioned2019-03-14T08:03:16Z-
dc.date.available2018-12-21T07:10:45Z
dc.date.available2019-03-14T08:03:16Z-
dc.date.issued2017-07-01en_US
dc.identifier.citationMicrochimica Acta. Vol.184, No.7 (2017), 1941-1950en_US
dc.identifier.issn14365073en_US
dc.identifier.issn00263672en_US
dc.identifier.other2-s2.0-85016442504en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016442504&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/42226-
dc.description.abstract© 2017, Springer-Verlag Wien. The authors report on the conjugation of monoclonal antibodies against the biomarker epithelial cell adhesion molecule (EpCAM) to silica nanoparticles doped with the dye Cy5 (Cy5-SiNPs). Conjugation was performed on the Cy5-SiNPs that were previously coated with a layer of protein G which serves as a linker controlling the orientation of the antibody. The conjugation method takes advantage of site specific interactions between the protein G and constant domains (Fc) of the antibody. The method warrants the antibody binding sites (Fab) to be faced outwards such that the conjugates maintain their affinity for binding the analyte (EpCAM). In vitro analysis by confocal fluorescence imaging and flow cytometry using analytical wavelengths comparable with the excitation and emission wavelength of Cy5-SiNPs at 643 and 662 nm, respectively. The result demonstrated the oriented conjugate to specifically bind to target cells (HT-29) with a sensitivity that is 12 times higher than that of conjugates prepared by conventional EDC coupling. In vivo fluorescence imaging of mice bearing the HT-29 tumor highlighted time-dependent accumulation of the oriented conjugates at the tumor site. As indicated by biodistribution studies hepatic excretion of the oriented probes occurs, however tumor fluorescence still remains for up to 14 days post injection. This research demonstrates that the oriented conjugates derived herein can improve target cell detection sensitivity and can be successfully applied in tumor imaging, which should drive further development of new classes of effective fluorescence contrast agents for cancer diagnostics.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85016442504&origin=inwarden_US
dc.subjectChemistryen_US
dc.titleOriented conjugation of antibodies against the epithelial cell adhesion molecule on fluorescently doped silica nanoparticles for flow-cytometric determination and in vivo imaging of EpCAM, a biomarker for colorectal canceren_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1007/s00604-017-2211-6en_US
Appears in Collections:Scopus 2016-2017

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