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dc.contributor.authorAlok Kafleen_US
dc.contributor.authorPranom Puchadapiromen_US
dc.contributor.authorSirikanya Plumworasawaten_US
dc.contributor.authorRieofarng Dontumpraien_US
dc.contributor.authorWaraporn Chan-onen_US
dc.contributor.authorSureemas Buatesen_US
dc.contributor.authorThewarach Lahaen_US
dc.contributor.authorBanchob Sripaen_US
dc.contributor.authorSutas Suttiprapaen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherKhon Kaen Universityen_US
dc.identifier.citationParasitology International. Vol.66, No.4 (2017), 426-431en_US
dc.description.abstract© 2016 Elsevier Ireland Ltd Protein 14-3-3s are abundant phospho-serine/threonine binding proteins, which are highly conserved among eukaryotes. Members of this protein family mediate metabolism and signal transduction networks through binding to hundreds of other protein partners. Protein 14-3-3s have been studied in other species of parasitic helminthes, but little is known about this protein in the carcinogenic liver fluke Opisthorchis viverrini. In this study, we identified and characterized protein 14-3-3s of O. viverrini. Seven protein 14-3-3 encoded sequences were retrieved from the O. viverrini genome database. Multiple alignment and phylogenetic analysis were performed. Two isoforms (protein 14-3-3 zeta and protein 14-3-3 epsilon) that have been previously found in the excretory-secretory (ES) products of O. viverrini were produced as recombinant protein in E. coli and the proteins were then used to immunize mice to obtain specific antibodies. Western blot analysis showed that both proteins were detected in all obtainable developmental stages of O. viverrini and the ES products. Immunolocalization revealed that both isoforms were expressed throughout tissues and organs except the gut epithelium. The highest expression was observed in testes especially in developing spermatocytes, suggesting their role in spermatogenesis. Prominent expression was also detected on tegumental surface of the parasite and on epical surface of bile duct epithelium indicates their additional role in host-parasite interaction. These findings indicate that protein 14-3-3s play important role in the life cycle of the parasite and might be involved in the pathogenesis of O. viverrini infection.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleIdentification and characterization of protein 14-3-3 in carcinogenic liver fluke Opisthorchis viverrinien_US
Appears in Collections:Scopus 2016-2017

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