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Title: Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein
Authors: Chutikarn Chaimayo
Tsuyoshi Hayashi
Andrew Underwood
Erin Hodges
Toru Takimoto
University of Rochester Medical Center
Mahidol University
National Center for Immunization and Respiratory Diseases
Keywords: Immunology and Microbiology
Issue Date: 1-May-2017
Citation: Virology. Vol.505, (2017), 23-32
Abstract: © 2017 Elsevier Inc. Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly.
ISSN: 10960341
Appears in Collections:Scopus 2016-2017

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