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dc.contributor.authorChutikarn Chaimayoen_US
dc.contributor.authorTsuyoshi Hayashien_US
dc.contributor.authorAndrew Underwooden_US
dc.contributor.authorErin Hodgesen_US
dc.contributor.authorToru Takimotoen_US
dc.contributor.otherUniversity of Rochester Medical Centeren_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNational Center for Immunization and Respiratory Diseasesen_US
dc.identifier.citationVirology. Vol.505, (2017), 23-32en_US
dc.description.abstract© 2017 Elsevier Inc. Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleSelective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 proteinen_US
Appears in Collections:Scopus 2016-2017

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