Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
Authors: Keisuke Tabata
Masaru Arimoto
Masashi Arakawa
Atsuki Nara
Kazunobu Saito
Hiroko Omori
Arisa Arai
Tomohiro Ishikawa
Eiji Konishi
Ryosuke Suzuki
Yoshiharu Matsuura
Eiji Morita
Osaka University
Hirosaki University
Nagahama Institute of Bio-Science and Technology
Dokkyo Medical University
Mahidol University
National Institute of Infectious Diseases
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 30-Aug-2016
Citation: Cell Reports. Vol.16, No.9 (2016), 2339-2347
Abstract: © 2016 The Author(s) Flavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. Systematic small interfering RNA (siRNA) screening revealed that release of both dengue virus and Japanese encephalitis virus was dramatically decreased by single depletion of TSG101 or co-depletion of specific combinations of ESCRT-III proteins, resulting in ≥1,000-fold titer reductions. By contrast, release was unaffected by depletion of some core ESCRTs, including VPS4. Reintroduction of ESCRT proteins to siRNA-depleted cells revealed interactions among ESCRT proteins that are crucial for flavivirus budding. Electron-microscopy studies revealed that the CHMP2 and CHMP4 proteins function directly in membrane deformation at the ER. Thus, a unique and specific subset of ESCRT contributes to ER membrane biogenesis during flavivirus infection.
ISSN: 22111247
Appears in Collections:Scopus 2016-2017

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.