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Title: Mass spectrometric analysis of host cell proteins interacting with dengue virus nonstructural protein 1 in dengue virus-infected HepG2 cells
Authors: Thanyaporn Dechtawewat
Atchara Paemanee
Sittiruk Roytrakul
Pucharee Songprakhon
Thawornchai Limjindaporn
Pa thai Yenchitsomanus
Sawanan Saitornuang
Chunya Puttikhunt
Watchara Kasinrerk
Prida Malasit
Sansanee Noisakran
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Chiang Mai University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 1-Sep-2016
Citation: Biochimica et Biophysica Acta - Proteins and Proteomics. Vol.1864, No.9 (2016), 1270-1280
Abstract: © 2016 Elsevier B.V. Dengue virus (DENV) infection is a leading cause of the mosquito-borne infectious diseases that affect humans worldwide. Virus–host interactions appear to play significant roles in DENV replication and the pathogenesis of DENV infection. Nonstructural protein 1 (NS1) of DENV is likely involved in these processes; however, its associations with host cell proteins in DENV infection remain unclear. In this study, we used a combination of techniques (immunoprecipitation, in-solution trypsin digestion, and LC–MS/MS) to identify the host cell proteins that interact with cell-associated NS1 in an in vitro model of DENV infection in the human hepatocyte HepG2 cell line. Thirty-six novel host cell proteins were identified as potential DENV NS1-interacting partners. A large number of these proteins had characteristic binding or catalytic activities, and were involved in cellular metabolism. Coimmunoprecipitation and colocalization assays confirmed the interactions of DENV NS1 and human NIMA-related kinase 2 (NEK2), thousand and one amino acid protein kinase 1 (TAO1), and component of oligomeric Golgi complex 1 (COG1) proteins in virus-infected cells. This study reports a novel set of DENV NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection.
ISSN: 18781454
Appears in Collections:Scopus 2016-2017

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