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Title: Increasing the triacylglycerol content in dunaliella tertiolecta through isolation of starch-deficient mutants
Authors: Anchalee Sirikhachornkit
Supachai Vuttipongchaikij
Anongpat Suttangkakul
Kittisak Yokthongwattana
Piyada Juntawong
Prayad Pokethitiyook
Kunn Kangvansaichol
Metha Meetam
Kasetsart University
Mahidol University
South Carolina Commission on Higher Education
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-May-2016
Citation: Journal of Microbiology and Biotechnology. Vol.26, No.5 (2016), 854-866
Abstract: © 2016 by The Korean Society for Microbiology and Biotechnology. The production cost of biodiesel from microalgae is still not competitive, compared with that of petroleum fuels. The genetic improvement of microalgal strains to increase triacylglycerol (TAG) accumulation is one way to reduce production costs. One of the most promising approaches is the isolation of starch-deficient mutants, which have been reported to successfully increase TAG yields. To date, such a stable mutant is not available in an oleaginous marine microalga, despite several advantages of using marine species for biodiesel production. Algae in the genus Dunaliella are known to tolerate high salt concentration and other environmental stresses. In addition, the cultivation processes for large-scale outdoor commercialization have been well established for this genus. In this study, Dunaliella tertiolecta was used to screen for starch-deficient mutants, using an iodine vapor-staining method. Four out of 20, 016 UV-mutagenized strains showed a substantial reduction of starch content. A significantly higher TAG content, up to 3-fold of the wild-type level, was observed in three of the mutants upon induction by nitrogen depletion. The carotenoid production and growth characteristics of these mutants, under both normal and oxidative stress conditions, were not compromised, suggesting that these processes are not necessarily affected by starch deficiency. The results from this work open up new possibilities for exploring Dunaliella for biodiesel production.
ISSN: 17388872
Appears in Collections:Scopus 2016-2017

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