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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/43090
Title: Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034
Authors: Bungonsiri Intra
Jirayut Euanorasetr
Takuya Nihira
Watanalai Panbangred
Mahidol University
Osaka University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Mar-2016
Citation: Applied Microbiology and Biotechnology. Vol.100, No.6 (2016), 2749-2760
Abstract: © 2015, Springer-Verlag Berlin Heidelberg. Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84959162007&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/43090
ISSN: 14320614
01757598
Appears in Collections:Scopus 2016-2017

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