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Title: Fabrication of protein microarrays for alpha fetoprotein detection by using a rapid photo-immobilization process
Authors: Sirasa Yodmongkol
Boonsong Sutapun
Verayuth Praphanphoj
Toemsak Srikhirin
Thomas Brandstetter
Jürgen Rühe
Mahidol University
Suranaree University of Technology
Rajanukul Institute
Universität Freiburg im Breisgau
Medical Genetics Center
Keywords: Biochemistry, Genetics and Molecular Biology;Computer Science;Engineering;Materials Science
Issue Date: 1-Mar-2016
Citation: Sensing and Bio-Sensing Research. Vol.7, (2016), 95-99
Abstract: © 2016 The Authors. In this study, protein microarrays based on sandwich immunoassays are generated to quantify the amount of alpha fetoprotein (AFP) in blood serum. For chip generation a mixture of capture antibody and a photoactive copolymer consisting of N,N-dimethylacrylamide (DMAA), methacryloyloxy benzophenone (MaBP), and Na-4-styrenesulfonate (SSNa) was spotted onto unmodified polymethyl methacrylate (PMMA) substrates. Subsequently to printing of the microarray, the polymer and protein were photochemically cross-linked and the forming, biofunctionalized hydrogels simultaneously bound to the chip surface by short UV- irradiation. The obtained biochip was incubated with AFP antigen, followed by biotinylated AFP antibody and streptavidin-Cy5 and the fluorescence signal read-out. The developed microarray biochip covers the range of AFP in serum samples such as maternal serum in the range of 5 and 100 ng/ml. The chip production process is based on a fast and simple immobilization process, which can be applied to conventional plastic surfaces. Therefore, this protein microarray production process is a promising method to fabricate biochips for AFP screening processes.
ISSN: 22141804
Appears in Collections:Scopus 2016-2017

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