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dc.contributor.authorSiriporn Kaewklomen_US
dc.contributor.authorJirayut Euanorasetren_US
dc.contributor.authorBungonsiri Intraen_US
dc.contributor.authorWatanalai Panbangreden_US
dc.contributor.authorRatchaneewan Aunpaden_US
dc.contributor.otherThammasat Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationInternational Journal of Peptide Research and Therapeutics. Vol.22, No.1 (2016), 93-100en_US
dc.description.abstract© 2015 Springer Science+Business Media New York. Plant defensins are small and basic antimicrobial peptides characterized by conserved cysteine stabilizing structure with α-helix and triple strand antiparallel β-sheet. In the present study, two novel defensin genes, designated as BhDef1 and BhDef2, was isolated from Brassica hybrid cv Pule, a native unexplored Brassicaceae species found in Thailand. The full-length cDNA of BhDef1 and BhDef2 were 240 and 258 bp encoding a 79 and 85 amino acid residues with 29 and 25 amino acid signal peptide at N-terminal, respectively. The putative BhDef1 and BhDef2 mature proteins showed significant similarity to other Brassicaceae defensins. Their secondary structure comprises of one α-helix and a triple stranded β-sheet stabilized by four disulphide bridges of eight cysteines. BhDef1 and BhDef2 also contain a highly conserved γ-core and α-core motif exhibiting antifungal activity against Colletotrichum gloeosporioides causing anthracnose disease. Six out of eight synthetic BhDef peptide derivatives showed antibacterial activity against both gram-positive bacteria and gram-negative bacteria used in this study. BhDef14, the derivative of BhDef1, showed the highest activity against two test pathogenic bacteria. This activity could probably due to a net positively charge and alpha-helical conformation which are known as the key determinant for the bacterial membrane disruption. To our knowledge, this is the first report on defensin genes isolated from B. hybrid cv Pule. The synthetic peptides designed from their sequences showed antifungal and antibacterial activity.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.titleAntimicrobial Activities of Novel Peptides Derived from Defensin Genes of Brassica hybrid cv Puleen_US
Appears in Collections:Scopus 2016-2017

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