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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/43149
Title: Intestinal mucosal changes and upregulated calcium transporter and FGF-23 expression during lactation: Contribution of lactogenic hormone prolactin
Authors: Kannikar Wongdee
Jarinthorn Teerapornpuntakit
Chanakarn Sripong
Asma Longkunan
Wasutorn Chankamngoen
Chutiya Keadsai
Kamonshanok Kraidith
Nateetip Krishnamra
Narattaphol Charoenphandhu
Mahidol University
Burapha University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 15-Jan-2016
Citation: Archives of Biochemistry and Biophysics. Vol.590, (2016), 109-117
Abstract: © 2015 Elsevier Inc. All rights reserved. As the principal lactogenic hormone, prolactin (PRL) not only induces lactogenesis but also enhances intestinal calcium absorption to supply calcium for milk production. How the intestinal epithelium res-ponses to PRL is poorly understood, but it is hypothesized to increase mucosal absorptive surface area and calcium transporter expression. Herein, lactating rats were found to have greater duodenal, jejunal and ileal villous heights as well as cecal crypt depths than age-matched nulliparous rats. Morphometric analyses in the duodenum and cecum showed that their mucosal adaptations were diminished by bromocriptine, an inhibitor of pituitary PRL release. PRL also upregulated calcium transporter expression (e.g., TRPV6 and PMCA1b) in the duodenum of lactating rats. Since excessive calcium absorption could be detrimental to lactating rats, local negative regulator of calcium absorption, e.g., fibroblast growth factor (FGF)-23, should be increased. Immunohistochemistry confirmed the upregulation of FGF-23 protein expression in the duodenal and cecal mucosae of lactating rats, consistent with the enhanced FGF-23 mRNA expression in Caco-2 cells. Bromocriptine abolished this lactation-induced FGF-23 expression. Additionally, FGF-23 could negate PRL-stimulated calcium transport across Caco-2 monolayer. In conclusion, PRL was responsible for the lactation-induced mucosal adaptations, which were associated with compensatory increase in FGF-23 expression probably to prevent calcium hyperabsorption.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84949486016&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/43149
ISSN: 10960384
00039861
Appears in Collections:Scopus 2016-2017

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