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Title: Scabraside D extracted from Holothuria scabra induces apoptosis and inhibits growth of human cholangiocarcinoma xenografts in mice
Authors: Kanjana Assawasuparerk
Rapeepun Vanichviriyakit
Charoonroj Chotwiwatthanakun
Saksit Nobsathian
Thanakorn Rawangchue
Boonsirm Wittayachumnankul
Department of Anatomy
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)
Mahidol University
Aquatic Animal Biotechnology Research Center
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2016
Citation: Asian Pacific Journal of Cancer Prevention. Vol.17, No.2 (2016), 511-517
Abstract: Scabraside D, a sulfated triterpene glycoside extract from sea cucumber Holothulia scabra, shows various biological activities, but effects on human cholangiocarcinoma cells have not previously been reported. In the present study, we investigated the activity of scabraside D against human cholangiocarcinoma (HuCCA) both in vitro and for tumor growth inhibition in vivo using a xenograft model in nude mice. Scabraside D (12.5-100 μg/mL) significantly decreased the viability and the migration of the HuCCA cells in a dose-dependent manner, with 50% inhibitory concentration (IC50) of 12.8 ± 0.05 μg/mL at 24 h. It induced signs of apoptotic cells, including shrinkage, pyknosis and karyorrhetic nuclei and DNA fragmentation on agarose gel electrophoresis. Moreover, by quantitative real-time PCR, scabraside D effectively decreased Bcl-2 while increasing Bax and Caspase-3 gene expression levels suggesting that the scabraside D could induce apoptosis in HuCCA cells. In vivo study demonstrated that scabraside D (1 mg/kg/day, i.p. for 21 days) significantly reduced growth of the HuCCA xenografts without adverse effects on the nude mice. Conclusively, scabraside D induced apoptosis in HuCCA cells and reduced the growth of HuCCA xenographs model. Therefore, scabraside D may have potential as a new therapeutic agent for cholangiocarcinoma.
ISSN: 15137368
Appears in Collections:Scopus 2016-2017

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