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|Title:||Structural and biochemical characterization of two heme binding sites on α<inf>1</inf>-microglobulin using site directed mutagenesis and molecular simulation|
Lena Wester Rosenlöf
Abdu I. Alayash
Center for Biologics Evaluation and Research
|Keywords:||Biochemistry, Genetics and Molecular Biology;Chemistry|
|Citation:||Biochimica et Biophysica Acta - Proteins and Proteomics. Vol.1864, No.1 (2016), 29-41|
|Abstract:||© 2015 Elsevier B.V. Background α1-Microglobulin (A1M) is a reductase and radical scavenger involved in physiological protection against oxidative damage. These functions were previously shown to be dependent upon cysteinyl-, C34, and lysyl side-chains, K(92, 118,130). A1M binds heme and the crystal structure suggests that C34 and H123 participate in a heme binding site. We have investigated the involvement of these five residues in the interactions with heme. Methods Four A1M-variants were expressed: with cysteine to serine substitution in position 34, lysine to threonine substitutions in positions (92, 118, 130), histidine to serine substitution in position 123 and a wt without mutations. Heme binding was investigated by tryptophan fluorescence quenching, UV-Vis spectrophotometry, circular dichroism, SPR, electrophoretic migration shift, gel filtration, catalase-like activity and molecular simulation. Results All A1M-variants bound to heme. Mutations in C34, H123 or K(92, 118, 130) resulted in significant absorbance changes, CD spectral changes, and catalase-like activity, suggesting involvement of these side-groups in coordination of the heme-iron. Molecular simulation support a model with two heme-binding sites in A1M involving the mutated residues. Binding of the first heme induces allosteric stabilization of the structure predisposing for a better fit of the second heme. Conclusions The results suggest that one heme-binding site is located in the lipocalin pocket and a second binding site between loops 1 and 4. Reactions with the hemes involve the side-groups of C34, K(92, 118, 130) and H123. General significance The model provides a structural basis for the functional activities of A1M: heme binding activity of A1M.|
|Appears in Collections:||Scopus 2016-2017|
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