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Title: Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
Authors: Yoshinori Utsumi
Maho Tanaka
Atsushi Kurotani
Takuhiro Yoshida
Keiichi Mochida
Akihiro Matsui
Manabu Ishitani
Supajit Sraphet
Sukhuman Whankaew
Thipa Asvarak
Jarunya Narangajavana
Kanokporn Triwitayakorn
Tetsuya Sakurai
Motoaki Seki
Centro Internacional de Agricultura Tropical
Mahidol University
Multidisciplinary Science Cluster
Japan Science and Technology Agency
Yokohama City University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jul-2016
Citation: Journal of Plant Research. Vol.129, No.4 (2016), 711-726
Abstract: © 2016, The Botanical Society of Japan and Springer Japan. Cassava anthracnose disease (CAD), caused by the fungus Colletotrichumgloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.
ISSN: 09189440
Appears in Collections:Scopus 2016-2017

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