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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/43414
Title: Extrinsic surface-enhanced Raman scattering detection of influenza A virus enhanced by two-dimensional gold@silver core-shell nanoparticle arrays
Authors: Kullavadee Karn-Orachai
Kenji Sakamoto
Rawiwan Laocharoensuk
Suwussa Bamrungsap
Sirirurg Songsivilai
Tararaj Dharakul
Kazushi Miki
National Institute for Materials Science Tsukuba
University of Tsukuba
Thailand National Science and Technology Development Agency
Mahidol University
Keywords: Chemical Engineering;Chemistry
Issue Date: 1-Jan-2016
Citation: RSC Advances. Vol.6, No.100 (2016), 97791-97799
Abstract: © 2016 Royal Society of Chemistry. A surface-enhanced Raman scattering (SERS) based biosensor using a direct immunoassay platform is demonstrated for influenza A detection. The nucleoprotein of influenza A virus, which is one of the most conserved and abundant structural proteins on the virion, was used as a target. In this study, highly sensitive biosensors were realized by combining specific recognition of antibody-antigen interactions and high signal enhancement of the SERS effect. SERS probes were fabricated by decorating PEGylated, 4,4′-thiobisbenzenethiol (TBBT)-labeled gold nanoparticles (NPs) with influenza A antibodies. To improve the sensitivity, a SERS immunoassay was performed on two-dimensional (2D) arrays of gold@silver core-shell (Au@Ag) NPs, which work as SERS substrates. The SERS signal of TBBT was utilized to detect the selective nucleoprotein-antibody recognition. The SERS signal was enhanced ∼4 times by using the SERS substrates instead of a flat Au film. These results indicate that using a well-tuned Au@Ag 2D array as a SERS substrate is an effective way of improving sensitivity of SERS-based biosensors. Our SERS immunoassay system revealed high selectivity and good reproducibility with a sample-to-sample variation of 4.6% (relative standard deviation). To demonstrate the applicability of our SERS immunoassay system to real biological samples, the detection of influenza A using infected allantoic fluid was also performed. The linear relation between the concentration of infected allantoic fluid and the SERS signal was obtained in the range of 5 to 56 TCID50 per mL (R2 = 0.96 for the TBBT Raman bands at 1565 cm-1) with the lowest detection limit of 6 TCID50 per mL. These findings demonstrated the potential of this SERS immunosensor platform for the highly sensitive and specific detection of target molecules in a complex matrix commonly found in clinical specimens.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84992187810&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/43414
ISSN: 20462069
Appears in Collections:Scopus 2016-2017

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