Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/43416
Title: Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing
Authors: Wanida Tangkawsakul
Toemsak Srikhirin
Kazunari Shinbo
Keizo Kato
Futao Kaneko
Akira Baba
Niigata University
Mahidol University
Keywords: Chemistry
Issue Date: 1-Jan-2016
Citation: International Journal of Analytical Chemistry. Vol.2016, (2016)
Abstract: © 2016 Wanida Tangkawsakul et al. In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test. We obtained the lowest detection limits of 1.58 × 105cells/ml for RBC-A and 3.83 × 105cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 108cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85008951175&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/43416
ISSN: 16878779
16878760
Appears in Collections:Scopus 2016-2017

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