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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/44673
Title: Ferrous and ferric differentially deteriorate proliferation and differentiation of osteoblast-like UMR-106 cells
Authors: Kornkamon Lertsuwan
Ketsaraporn Nammultriputtar
Supanan Nanthawuttiphan
Supathra Phoaubon
Jomnarong Lertsuwan
Jirawan Thongbunchoo
Kannikar Wongdee
Narattaphol Charoenphandhu
Chulabhorn Research Institute
Mahidol University
Burapha University
Academy of Science
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Materials Science
Issue Date: 1-Oct-2018
Citation: BioMetals. Vol.31, No.5 (2018), 873-889
Abstract: © 2018, Springer Nature B.V. The association between iron overload and osteoporosis has been found in many diseases, such as hemochromatosis, β-thalassemia and sickle cell anemia with multiple blood transfusion. One of the contributing factors is iron toxicity to osteoblasts. Some studies showed the negative effects of iron on osteoblasts; however, the effects of two biological available iron species, i.e., ferric and ferrous, on osteoblasts are elusive. Since most intracellular ionized iron is ferric, osteoblasts was hypothesized to be more responsive to ferric iron. Herein, ferric ammonium citrate (FAC) and ferrous ammonium sulfate (FAS) were used as ferric and ferrous donors. Our results showed that both iron species suppressed cell survival and proliferation. Both also induced osteoblast cell death consistent with the higher levels of cleaved caspase 3 and caspase 7 in osteoblasts, indicating that iron induced osteoblast apoptosis. Iron treatments led to the elevated intracellular iron in osteoblasts as determined by atomic absorption spectrophotometry, thereby leading to a decreased expression of genes for cellular iron import and increased expression of genes for cellular iron export. Effects of FAC and FAS on osteoblast differentiation were determined by the activity of alkaline phosphatase (ALP). The lower ALP activity from osteoblast with iron exposure was found. In addition, ferric and ferrous differentially induced osteoblastic and osteoblast-derived osteoclastogenic gene expression alterations in osteoblast. Even though both iron species had similar effects on osteoblast cell survival and differentiation, the overall effects were markedly stronger in FAC-treated groups, suggesting that osteoblasts were more sensitive to ferric than ferrous.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049999197&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/44673
ISSN: 15728773
09660844
Appears in Collections:Scopus 2018

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