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Title: Presence of Penaeus monodon densovirus in the ovary of chronically infected P. monodon subadults
Authors: Sudini Fernando
Pongsopee Attasart
Salin R. Krishna
Boonsirm Withyachumnarnkul
Rapeepun Vanichviriyakit
Uva Wellassa University
Mahidol University
Asian Institute of Technology Thailand
Prince of Songkla University
AquaAcademy Farm
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)
Keywords: Agricultural and Biological Sciences
Issue Date: 14-Aug-2018
Citation: Diseases of Aquatic Organisms. Vol.129, No.3 (2018), 183-191
Abstract: © Inter-Research 2018. Shrimp infected with Penaeus monodon densovirus (PmoDNV) usually display no specific gross signs, but heavy infections can kill postlarvae and retard juvenile growth. In the present study, samples of hepatopancreas, feces, gonads and hemolymph were isolated from male and female P. monodon subadults chronically infected by PmoDNV. Each sample of hepatopancreas and gonad was divided into 2 parts: one for PmoDNV detection by polymerase chain reaction (PCR), and the other for routine histology and immunohistochemistry. The frequency of positive findings via PCR assays was 92% in the hepatopancreas, 57% in feces, 50% in ovary, 35% in hemolymph and 0% in the testis. Using the densitometric value (DV) of the specific band for PmoDNV relative to that of the β-actin gene as an index of the viral load in the samples, no significant differences were observed among sample types and sexes. Hematoxylin-eosin staining of infected hepatopancreas revealed typical PmoDNV inclusions in the nuclei of infected cells. The ovaries with high DV (>1) contained various types of inclusions along the row of the follicular cells or possibly in the connective tissue cells surrounding the oocytes. Using immunohistochemistry with specific probes to detect PmoDNV proteins, a positive reaction was observed in viral inclusions found in infected hepatopancreas and in ovaries with high DV, specifically in the ovarian capsule, hemolymph, oocytes and nuclear inclusions. These results suggest that the localization of PmoDNV in P. monodon is not confined to the hepatopancreas, but rather that the virus can also occur in the ovary; hence, trans-ovarian, vertical transmission of the virus is highly possible.
ISSN: 16161580
Appears in Collections:Scopus 2018

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