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dc.contributor.authorJiraporn Srisalaen_US
dc.contributor.authorRapeepan Pukmeeen_US
dc.contributor.authorRobin McIntoshen_US
dc.contributor.authorSudharma Choosuken_US
dc.contributor.authorOrnchuma Itsathitphaisarnen_US
dc.contributor.authorTimothy W. Flegelen_US
dc.contributor.authorKallaya Sritunyalucksanaen_US
dc.contributor.authorRapeepun Vanichviriyakiten_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.contributor.otherCharoen Pokphand Foods Public Company Limiteden_US
dc.identifier.citationAquaculture. Vol.493, (2018), 93-99en_US
dc.description.abstract© 2018 Elsevier B.V. Spiroplasma eriocheiris was first named as the cause of tremor disease in the Chinese mitten crab Eriocheir sinensis in China in 2003. It was subsequently reported from China to infect also the crayfish Procambarus clarkii, the giant river prawn Macrobrachium rosenbergii, the oriental prawn Macrobrachium nipponensis and the whiteleg shrimp Penaeus vannamei. Unfortunately, none of these publications give clear pictures of the histopathology caused by the pathogen, even though this is one of the first diagnostic procedures often performed for newly reported diseases. Here we describe distinctive histological lesions caused in the hepatopancreatic tissue of M. rosenbergii infected with S. eriocheiris. In the hepatopancreas (HP), these consist of basophilic or mixed basophilic/eosinophilic lesions in cells confined to the interstitial spaces of the HP and not involving the hepatopancreatic tubule epithelial cells. Examination of the lesions by transmission electron microscopy revealed the presence of colonies of intracellular, un-walled bacterial cells of variable shape (but many crescent shaped) and approximately 150 nm in diameter. PCR using group-specific primers for the SSU rRNA followed by cloning and sequencing revealed 99.9% sequence identity to the matching sequence of S. eriocheiris. PCR tests for presence of S. eriocheiris in hepatopancreatic tissue samples gave positive test results, and in situ hybridization assays using a probe derived from the PCR product gave positive signals in the interstitial spaces of the HP, corresponding to the regions of the distinctive histological lesions. Less severe lesions containing cells with eosinophilic/basophilic inclusions were also found and confirmed by in situ hybridization in heart muscle, skeletal muscle, gills, connective tissue and the ventral nerve cord. Recognition of the distinctive histological lesions in interstitial spaces of the hepatopancreatic tissue of M. rosenbergii by histological analysis can be used as a strong presumptive indication of infection by S. eriocheiris and may help in the control of this rapidly-spreading, emerging pathogen.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleDistinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergiien_US
Appears in Collections:Scopus 2018

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